Affordable and reliable plant sap-mediated template preparation for the detection of various phytopathogens by PCR assay

Sahana, A. B. and Nagaraja, H. and Maheshwar, P. K. and Sreenivasa, M. Y. and Nagendra Prasad, M. N. and Adkar-Purushothama, C. R. (2014) Affordable and reliable plant sap-mediated template preparation for the detection of various phytopathogens by PCR assay. Phytoparasitica, 42 (4). pp. 519-527. ISSN 0334-2123

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Official URL: http://dx.doi.org/10.1007/s12600-014-0389-2

Abstract

Plant pathogens like ‘Ca. Liberibacter’, phytoplasma, viruses and viroids cause diseases to almost all economically important plants. A simple and fast sap-mediated polymerase chain reaction (PCR) method for the detection of these pathogens infecting various plant hosts is described in the present study. Sap from selected plants was drawn aseptically on parafilm, from the mid-rib of young leaves. Depending on the type of host plant, sap was diluted to optimal concentration before PCR analysis. ‘Ca. Liberibacter’, Citrus tristeza virus (CTV) and Citrus exocortis viroid (CEVd) infecting citrus, and ‘Ca. Phytoplasma’ infecting pepper and sandal trees were tested by sap-mediated PCR. The reliability of this procedure was evaluated by comparing the findings with previously described protocols. The sap-mediated nucleic acid template preparation for PCR assay is devoid of laborious nucleic acid extraction and expensive chemicals. Hence the present method is rapid, economical and so can be employed for diagnosis of large number of plant samples.

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Phytoparasitica [Field not mapped to EPrints]
Uncontrolled Keywords: Ca. Liberibacter, Citrus tristeza virus, Phytoplasma, RT-PCR, Viroids
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: Constituent Colleges > Yuvaraja's College Mysore > Microbiology
PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Praveen Kumari B.L
Date Deposited: 07 Jun 2014 09:49
Last Modified: 25 Apr 2015 10:14
URI: http://eprints.uni-mysore.ac.in/id/eprint/16831

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