Suresh, C. and Dubey, A. K. and Kini, R. and Umesh Kumar, S. and Karanth, N. G. K. (1999) Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels. Electrophoresis, 20 (3). pp. 483-485.
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Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels.pdf Restricted to Registered users only Download (63kB) | Request a copy |
Abstract
A procedure to detect raw and gelatinized starch activities of glucoamylase on isoelectric focusing (IEF) gels by using 2, 3, 5-triphenyltetrazolium chloride is described. The reagent reacts with the reducing group of glucose released by glucoamylase from the substrate starch. Using the reaction, production of glucoamylase by Aspergillus niger was detected on 10% IEF gels within a pH range of 2.5-9.5. Since the method can detect raw and gelatinized starch activities of glucomylase associated with 1 mu g protein, it will be useful for enzyme engineering studies that involve screening of various mutations.
| Item Type: | Article |
|---|---|
| Subjects: | B Life Science > Botany |
| Divisions: | Department of > Botany |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 16 Jun 2021 04:51 |
| Last Modified: | 22 Jul 2022 05:53 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/16819 |
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