Rudrammaji, L. M. S. and Veerabasappa Gowda, T. (1998) Purification and characterization of three acidic, cytotoxic phospholipases A(2) from Indian cobra (Naja naja naja) venom. Toxicon, 36 (6). pp. 921-932.
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Three acidic phospholipases A(2) (NN-I-2c-PLA(2), NN-I-2d-PLA(2) and NN-I-2e-PLA(2)) were purified by successive chromatography of Indian cobra (Naja naja naja) venom on CM-Sephadex C-25, Sephadex G-50 and QAE-Sephadex A-25 columns. They have molecular weights of 13 000-14 500 by sodium dodecyl sulphatepolyacrylamide gel electrophoresis. They showed tryptophan specific fluorescence emission spectra (similar to 345 nm). All the three phospholipases A(2) were enzymatically highly active with specific activities 9-17 mu mol min(-1) mg(-1) They were non-lethal to mice when injected intraperitoneally in doses up to 10 mg kg(-1) body weight. They induced edema in mouse foot pads and were cytotoxic to Ehrlich ascites tumour cells. They did not exhibit direct haemolytic and anticoagulant activities. (C) 1998 Elsevier Science Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Subjects: | D Physical Science > Physics |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 07 Jun 2021 04:53 |
| Last Modified: | 12 Jul 2022 06:19 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/16715 |
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