Development of ELISA for the detection of Ralstonia solanacearum in tomato: its application in seed health testing

Rajeshwari, N. and Shylaja, M. D. and Krishnappa, M. and Shetty, H. S. and Mortensen, C. N. and Mathur, S. B. (1998) Development of ELISA for the detection of Ralstonia solanacearum in tomato: its application in seed health testing. World Journal of Microbiology and Biotechnology, 14 (5). pp. 697-704.

Text Development of ELISA for the detection of Ralstonia solanacearum in tomato - its application in seed health testing.pdf Restricted to Registered users only Download (141kB) | Request a copy

Abstract

Bacterial wilt caused by Ralstonia (formerly Pseudomonas) solanacearum is worldwide in distribution, It is one of the most destructive bacterial diseases of economically important crops. The serological assays so far developed for the detection of R. solanacearum were able to provide information as to the presence or absence of the pathogen in soil and plant materials. However, they could not discriminate between virulent and avirulent strains of the pathogen and were not specific to strains and races. In the present investigation, virulent bacterial cells (encapsulated with mucin) from tomato seeds were used as antigen and polyclonal antisera were developed in rabbit using a classical immunization protocol. Antisera thus developed were examined for the antibody titre, sensitivity, specificity, rapidity and the efficacy of the antibody in identifying the virulent and avirulent strains of the pathogen and the potential for application of this assay to the screening of infected plant materials and seeds. Our results indicate that the anti-tomato R. solanacearum: (i) has a good antibody titre of 1:10,000; (ii) can detect as few as 100 bacterial cells/ml; (iii) is tomato-specific (it reacted with tomato A. solanacearum, and not with isolates from chilli or eggplant); (iv) is reactive to all isolates of R. solanacearum from tomato; (v) is not cross-reactive with non-pseudomonads; (vi) is virulent strain-specific as it recognizes the virulent exopolysaccharide component as an antigenic determinant; (vii) reactivity could be correlated well with the degree of infection in tomato seeds and plant materials. The enzyme linked immunosorbent assay developed is sensitive, specific and rapid, therefore suitable for the detection of R. solanacearum isolates from tomato seeds during routine assays.

Item Type:	Article
Subjects:	B Life Science > Botany
Divisions:	Department of > Botany
Depositing User:	Users 23 not found.
Date Deposited:	10 Jun 2021 05:14
Last Modified:	13 Dec 2022 05:52
URI:	http://eprints.uni-mysore.ac.in/id/eprint/16695

Actions (login required)

View Item