Molecular identification of aiiA homologous gene from endophytic Enterobacter species and in silico analysis of putative tertiary structure of AHL-lactonase

Rajesh, P. S. and Rai, V. Ravishankar (2014) Molecular identification of aiiA homologous gene from endophytic Enterobacter species and in silico analysis of putative tertiary structure of AHL-lactonase. Biochemical and Biophysical Research Communications, 443 (1). pp. 290-295.

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Official URL: http://dx.doi.org/10.1016/j.bbrc.2013.11.101

Abstract

The aiiA homologous gene known to encode AHL- lactonase enzyme which hydrolyze the N -acylhomoser- ine lactone (AHL) quorum sensing signaling molecules produced by Gram negative bacteria. In this study, the degradation of AHL molecules was determined by cell-free lysate of endophytic Enterobacter species. The percentage of quorum quenching was confirmed and quantified by HPLC method ( p < 0.0001). Ampli- fication and sequence BLAST analysis showed the presence of aiiA homologous gene in endophytic Enter- obacter asburiae VT65, Enterobacter aerogenes VT66 and Enterobacter ludwigii VT70 strains. Sequence alignment analysis revealed the presence of two zinc binding sites, "HXHXDH" motif as well as tyrosine residue at the position 194. Based on known template available at Swiss-Model, putative tertiary struc- ture of AHL-lactonase was constructed. The result showed that novel endophytic strains of Enterobacter genera encode the novel aiiA homologous gene and its structural importance for future study.

Item Type: Article
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Chandrappa
Date Deposited: 03 Mar 2014 06:18
Last Modified: 18 Dec 2015 11:45
URI: http://eprints.uni-mysore.ac.in/id/eprint/16655

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