Prasad, B. N. and Kemparaju, K. and Bhatt, Subramanya K. G. and Veerabasappa Gowda, T. (1996) A platelet aggregation inhibitor phospholipase A(2) from Russell's viper (Vipera russelli) venom: Isolation and characterization. Toxicon, 34 (10). pp. 1173-1185. ISSN 0975-072X
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Yipera russelli venom is reported to contain multiple forms of phospholipase A(2) (PLA(2)). Three PLA(2)s (VRV-PL-V, VRV-PL-VI and VRV-PL-VIIIa) have been purified and characterized in this laboratory. A PLA(2) (VRV-PL-IIIb) inhibiting platelet aggregation was purified from the same venom in two steps involving CM-Sephadex C-25 column chromatography followed by gel filtration on Sephadex G-50. VRV-PL-IIIb is a basic (pi 7.3-7.7) isoenzyme, with a mel. wt between 14,000 and 15,000. It induced neurotoxic symptoms in experimental mice with an i.p. LD(50) of 5.2 mg/kg body weight. VRV-PL-IIIb inhibits ADP-induced platelet aggregation in a dose-dependent manner. It induced oedema in the foot pads of mice and is devoid of anticoagulant, myotoxic and direct haemolytic activities. Antibodies to VRV-PL-IIIb showed a single precipitin line against the antigen as well as whole venom. Copyright (C) 1996 Elsevier Science Ltd
| Item Type: | Article |
|---|---|
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 23 May 2021 05:28 |
| Last Modified: | 01 Jul 2022 08:40 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/16556 |
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