Effect of capsaicin on phospholipase A2 activity and superoxide generation in macrophages

Savitha, G. and Bharathi P. Salimath (1995) Effect of capsaicin on phospholipase A2 activity and superoxide generation in macrophages. Nutrition Research, 15 (10). pp. 1417-1427. ISSN 0271-5317

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Official URL: https://doi.org/10.1016/0271-5317(95)02014-M


The mechanism of inhibition of Ca2+ - triggered phospholipase A(2) (PLA(2)) activity and respiratory burst in macrophages by shown that capsaicin inhibits calcium-ionophore stimulated pro-inflammatory responses in macrophages such as generation of superoxide anion, PLA(2) activity (IC50 = 20 uM) and membrane liquid peroxidation (IC50 = 10 uM). Both capsaicin and PLA(2) and dose dependent manner. Arachidonic acid, linoleic acid and SDS restored capsaicin inhibited respiratory burst. Capsaicin and known PLA(2) inhibitors, dexamethasone and indomethacin, inhibited Ca2+-dependent PLA(2) activity in vitro from macrophages. Inhibition of PLA(2) activity by capsaicin is independent of Ca2+ and substrate concentration. Fluorescence studies studies suggest that capsaicin interacts directly with partially purified macrophage PLA(2). Finally, the antioxidant property of capsaicin was comparable to that of butylated hydroxy toludine (BHT). Taken together these results show that capsaicin an antiinflammatory agent with potential clinical application.

Item Type: Article
Subjects: C Chemical Science > Biochemistry
Depositing User: Users 23 not found.
Date Deposited: 21 May 2021 04:48
Last Modified: 20 Jun 2022 11:19
URI: http://eprints.uni-mysore.ac.in/id/eprint/16487

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