Upadhyaya, G. and Shivanna, M. B. and Prakash, H. S. and Shetty, H. S. (1992) A novel approach to the establishment of dual cultures of pearl millet and Sclerospora graminicola. Plant Cell, Tissue and Organ Culture, 31 (3). pp. 203-206.
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A novel approach to the establishment of dual cultures of pearl millet and Sclerospora graminicola.pdf Restricted to Registered users only Download (326kB) | Request a copy |
Abstract
Dual cultures were successfully established using malformed florets of pearl millet infected with Sclerospora graminicola, the downy mildew pathogen. A higher proportion (86%) of calli from malformed florets formed dual cultures on Murashige and Skoog's (MS) medium with 2 mg l-1 of 2,4-dichlorophenoxy acetic acid (2,4-D), Compared to shoot tips (25%). Fungal mycelium covered the entire surface of the callus within 30 days of placement of explants on the MS medium with 2 mg l-1 of 2,4-D. The infected calli also differentiated and produced plantlets when transferred to MS medium without 2,4-D.
| Item Type: | Article |
|---|---|
| Subjects: | B Life Science > Botany |
| Divisions: | Department of > Botany |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 18 May 2021 07:11 |
| Last Modified: | 13 Dec 2022 06:04 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/16407 |
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