In vitro regeneration from excised leaves of Flaveria trinervia (Sprengel) C. Mohr

Sudarshana, M.S. and Shanthamma, C. (1991) In vitro regeneration from excised leaves of Flaveria trinervia (Sprengel) C. Mohr. Plant Cell, Tissue and Organ Culture, 27 (3). pp. 297-302.

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Flaveria trinervia (Compositae) leaves are used for the treatment of jaundice and fever. From the leaf callus cultures regeneration of plantlets has been achieved. The results showed that BAP greatly stimulated the bud formation in concentrations ranging from 2-5 mg l-1 than at very low concentrations (0.2-1.0 mg l-1). Roots developed on the regenerated shoots, over a range of treatments, but were most prolific in the medium containing 1 mg l-1 IAA. Histological observations revealed that cultured spongy cells of the mesophyll were greatly enlarged and underwent repeated cell divisions leading to the formation of hard nodular callus from which shoot buds differentiated. The shoots obtained were readily rooted and transplanted into glass houses. Cytological studies of the callus showed abnormalities such as bridges, endomitosis and multinucleolate conditions. Root tip squashes of the regenerated plants showed no variations and were diploid in chromosome number.

Item Type: Article
Subjects: B Life Science > Botany
Divisions: Department of > Botany
Depositing User: Users 23 not found.
Date Deposited: 12 May 2021 07:48
Last Modified: 12 May 2021 07:48

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