Marathe, G. K. and D'Souza, C. J. M. (1993) Production of Oxygen Free-Radicals By Ehrlich Ascites Tumor-Cells - Effect of Lipids. Mediators of Inflammation, 2 (1). pp. 53-57.
![[img]](http://eprints.uni-mysore.ac.in/style/images/fileicons/text.png) |
Text
Production of Oxygen Free-Radicals By Ehrlich Ascites Tumor-Cells - Effect of Lipids.pdf Restricted to Registered users only Download (1MB) | Request a copy |
Abstract
PHORBOL-12-myristate-13-acetate (PMA), calcium ionophore A23187 and platelet activating factor (PAF) stimulated the generation of oxygen free radicals (nitro-blue tetrazolium reduction) in Ehrlich ascites tumour (EAT) cells. PAF was effective at an optimal concentration of 4 muM, but was inhibited by BN 52021, a specific PAF antagonist. Lyso-PAF was ineffective. Inclusion of different lipids during incubation prior to the addition of PAF, resulted in the activation/inhibition of free radical generation. Among the phospholipids at a concentration of 50 mug/ml, the order of activation was phosphatidylserine > phosphatidylglycerol > phosphoinositides > phosphatidylinositol > phosphatidylethanolamine. Phosphatidylcholine was not effective, while sphingolipids were inhibitory. In addition, Ehrlich ascites tumour cells grown in mice under marginal vitamin A deficiency, showed an augmented production of free radicals compared to control cells. This was suppressed by exogenous addition of vitamin A or superoxide dismutase. These results suggest that membrane lipids and dietary factors like vitamin A probably function as physiological modulators in regulating the free radical generation.
| Item Type: | Article |
|---|---|
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 04 May 2021 09:00 |
| Last Modified: | 09 May 2023 07:21 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/16332 |
Actions (login required)
 |
View Item |
