Appaiah, K. A. A. and Shaw, D. M. and Karanth, N. G. K. (1993) Selective plating medium for enumeration and recovery of a hexachlorocyclohexane‐degrading Pseudomonas Ptm+ strain from soil. Letters in Applied Microbiology, 16 (6). pp. 287-290.
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A double selective plating medium has been developed to enumerate a Pseudomonas Ptm+ strain capable of assimilating hexachlorocyclohexane (HCH). The ability of the isolate to utilize HCH and/or streptomycin as sole sources of carbon was chosen to impart selectivity. In trials when serial 10-fold soil dilutions were spread on HCH plus streptomycin mineral agar and incubated, the normal microflora did not grow. When soil containing an HCH degrading Pseudomonas was cultured, only the target colonies developed. This double selective plating method is suitable for environmental monitoring of HCH scavengers as the medium is sensitive, specific and practical.
| Item Type: | Article |
|---|---|
| Subjects: | B Life Science > Botany |
| Divisions: | Department of > Botany |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 06 May 2021 06:20 |
| Last Modified: | 26 May 2022 10:09 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/16308 |
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