Basavarajappa, B. S. and Guru, S. C. and Veerabasappa Gowda, T. (1993) Immunochemical cross-reactivity of neurotoxic phospholipase A2 enzymes from Indian cobra (Naja naja naja) venom using polyclonal antibodies. Toxicon, 31 (9). pp. 1167-1177.
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Rabbit antibodies were prepared against purified phospholipase A2 (NN-XI(a)-PLA2) from Indian cobra (Naja naja naja) venom. The PLA2 has haemolytic, neurotoxic, myotoxic, cytotoxic and oedema-inducing activities apart from the catalytic activity. The immunological cross-reactivity of structurally similar neurotoxic PLA2s was investigated using enzyme-linked immunosorbent assay (ELISA) and immunodiffusion. Anti-NN-XI(a)-PLA2 IgG cross-reacted with other purified neurotoxic PLA2S from the same venom. Immunochemical cross-reactions of anti-NN-XI(a)-PLA2 IgG with NN-XI(a)-PLA2, NN-XI(b)-PLA2, NN-XIII-PLA, NN-IV(b1)-PLA2 and NN-V(b)-PLA, were shown by a very high ELISA titre and a single precipitin band on double immunodiffusion agarose plates. The catalytic activity of these PLA2s was inhibited dose-dependently by anti-NN-XI(a)-PLA2 IgG but was unable to neutralize lethality and neurotoxic symptoms in experimental animals injected with neurotoxic PLA2. Anti-NN-XI(a)-PLA, IgG fails to neutralize myotoxicity and oedema-inducing activities of NN-XI(a)-PLA2 and NN-XIII-PLA2. Anti-NN-XI(a)-PLA2 IgG inhibited cytotoxic effects of NN-XI(a)-PLA2 dose-dependently, but failed to inhibit NN-XIII-PLA2-induced cytotoxicity. Direct haemolytic activity of NN-XI(a)-PLA2 and NN-XIII-PLA2 was inhibited dose-dependently by these antibodies. The results indicate the presence of separate catalytic and pharmacologic site(s).
| Item Type: | Article |
|---|---|
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 06 May 2021 08:33 |
| Last Modified: | 12 Jul 2022 09:37 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/16294 |
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