Cellulysin induces downy mildew disease resistance in pearl millet driven through defense response.

Pushpalatha, H. G. and Sudisha, J. and Shetty, H. S. (2013) Cellulysin induces downy mildew disease resistance in pearl millet driven through defense response. European Journal of Plant Pathology, 137 (4, Cop). pp. 707-717. ISSN 0929-1873

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Abstract

The responses to cellulysin as an immune inducer in pearl millet that confers downy mildew resis- tance mediated through lipoxygenase (LOX), a jasmonate-dependent enzyme involved in defence sig- nalling, are discussed in this paper. The susceptible pearl millet cultivar 7042S was treated with cellulysin at 10, 15, 20, 30 and 50 μ g/ml concentrations. All tested concentrations showed enhanced seed germination and seedling vigour when compared with the untreated con- trol. Maximum seed germination of 92 % and seedling vigourwas obtainedfollowing 20 μ g/mlcellulysintreat- ment. Significant ( P <0.05) downy mildew disease pro- tection of 67 % and 71 % was observed when cellulysin was used at 20 μ g/ml under greenhouse and field con- ditions, respectively. Further studies showed that the resistance induced by cellulysin treatment in pearl millet plant was systemic, required a minimum of 4 days to achieve maximum resistance development after patho- gen inoculation seedling inoculation (five-day-old), and twas sustained throughout the plant ’ s life. Plants raised from cellulysin-treated seeds and challenge inoculated at tillering (25-day-old) and inflorescence (45-day-old) showed persistence in resistance till the end of the crop period. A notable increase in LOX activity was ob- served in all the tested concentrations of cellulysin in plants inoculated with the pathogen at 24 h, compared to the control. However, a maximum 6-fold increase in LOX activity was noticed using a cellulysin concentra- tion of 20 μ g/ml 48 hours post inoculation. In contrast, glucanase (GLU) activity was high in control inoculated seedlings, but was low in cellulysin treated samples at all time intervals. The opt imal cellulysin treatment (20 μ g/ml) provided enhanced vegetative and reproduc- tive parameters that resulted in higher yield compared to the untreated control

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2013/// [EPrints field already has value set] M3 - 10.1007/s10658-013-0281-9 [Field not mapped to EPrints] JA - Eur. J. Plant Pathol. [Field not mapped to EPrints]
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Kodandarama
Date Deposited: 21 Jan 2014 07:54
Last Modified: 16 Sep 2014 06:09
URI: http://eprints.uni-mysore.ac.in/id/eprint/16232

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