Kemparaju, K. and Nijaguna Prasad, B. and Veerabasappa Gowda, T. (1994) Purification of a basic phospholipase A2 from Indian saw-scaled viper (Echis carinatus) venom: characterization of antigenic, catalytic and pharmacological properties. Toxicon, 32 (10). pp. 1187-1196.
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A major basic phospholipase A(2) was purified from the Indian saw-scaled viper (Echis carinatus) venom by the combination of column chromatography and electrophoresis. The purified phospholipase A(2) (EC-IV-PLA(2)) has a mel. wt of 14,000 by SDS-PAGE. It is a basic protein with a pI value between 7.2 and 7.6, and has a fluorescence emission maxima at 340 nm. It induces neurotoxicity and oedema in mice with an i.p. LD(50) Of 5 mg/kg body weight. It is devoid of direct haemolytic, myotoxic, cytotoxic and anticoagulant activities. Rabbit polyclonal antibodies prepared against EC-IV-PLA(2) inhibited the in vitro enzymatic activity dose dependently, but did not neutralize the toxic effects of EC-IV-PLA(2) in experimental animals.
| Item Type: | Article |
|---|---|
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 03 May 2021 06:32 |
| Last Modified: | 07 Jul 2022 09:51 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/16227 |
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