Métodos sensitivos para la determinación espectrofotométrica de los compuestos antineoplásic

Nagaraja, P. and Vasantha, R. A. and Yathirajan, H. S. (2002) Métodos sensitivos para la determinación espectrofotométrica de los compuestos antineoplásic. Ars Pharmaceutica, 43 (3-4). pp. 121-133.

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Abstract

Sensitive, rapid and simple spectrophotometric methods for the determination of anticancer drugs, vinblastine sulphate (VBS) and vincristine sulphate (VCS) have been described for the first time. The first method is based on the azo coupling reaction of VBS and VCS with diazotised 4-nitroaniline (4-NA) resulting in yellow coloured products having absorbance maxima at 440 nm and 430 nm respectively. The yellow products of both VBS and VCS are stable for 1h at room temperature and Beer’s law is obeyed in the concentration range of 0.5-18 mg ml-1 for VBS and 0.5-10 mg ml- 1 for VCS. The second method describes the reaction of VBS and VCS with iron(III) and 2,4,6-tri(2-pyridyl)-1,3,5- triazine (TPT) to produce purple coloured products having absorption maximum at 590 nm and the products are stable for 48h. The Beer’s law for this method is obeyed in the concentration range of 0.2-4.0 mg ml-1 for VBS and 0.2-5.0 mg ml-1 for VCS. Both the methods are successfully employed for the determination of VBS and VCS in pharmaceutical preparations and the results compare favourably with official method. Commonly used excipients as additives do not interfere in the analysis. Some optical characteristics and precision data have been reported.

Item Type: Article
Additional Information: Unmapped bibliographic data: JO - Ars Pharmaceutica [Field not mapped to EPrints]
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Ambika P Patil
Date Deposited: 17 Oct 2013 10:11
Last Modified: 17 Oct 2013 10:26
URI: http://eprints.uni-mysore.ac.in/id/eprint/15788

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