Studies on Anti-oxidant activity of Tinospora cordifolia (Miers.) Leaves using in vitro models

Ramya Premanath, and Lakshmi Devi, N. (2010) Studies on Anti-oxidant activity of Tinospora cordifolia (Miers.) Leaves using in vitro models. Journal of American Science, 6 (10).

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Abstract

Plants produce a diverse range of bioactive molecules, making them a rich source of different types of medicines. A regular and widespread use of herbs throughout the world has increased serious concern over their quality, safety and efficacy. Thus, a proper scientific evidence or assessment has become the criteria for acceptance of herbal health claims. In the present study, we examined the anti-oxidant effects of leaves of Tinospora cordifolia. Dried and powered leaves of T. cordifolia were extracted with hexane, chloroform, methanol, ethanol and water. Total phenolic and flavonoid contents of different solvent extracts were determined. Of the different solvent extracts, ethanol extract had the highest phenol and flavonoid content of 5.1±0.25 mg/g and 0.52±0.02 mg/g respectively. Antioxidant assays were carried out by using different in vitro models such as total reducing power, total antioxidant activity, lipid peroxidation inhibitory activity, DPPH radical scavenging activity and superoxide radical scavenging activity. Ethanol extract showed the highest total antioxidant activity of 41.4±0.45 µM Fe(II)/g. The EC50 values of ethanol extract for lipid peroxidation inhibitory activity and DPPH radical scavenging activity was found to be 0.1 and 0.5 mg/ml respectively. The anti-oxidant activities of other solvent extracts were poor when compared to the ethanol extract. These results suggest that, the active antioxidant compounds are better extracted in ethanol and there is a direct correlation between the total polyphenols extracted and its anti-oxidant activity. The in vitro anti-oxidant activity of T. cordifolia justifies the ethno medical use of this plant

Item Type: Article
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Dhruvakumar
Date Deposited: 17 Oct 2013 07:34
Last Modified: 30 Nov 2015 11:08
URI: http://eprints.uni-mysore.ac.in/id/eprint/15781

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