Protective Effect of Tamarind Seed Coat Ethanol Extract on Eryptosis Induced by Oxidative Stress

Kengaiah, J. and Nandish, S. K. M. and Ramachandraiah, C. and Chandramma and Shivaiah, A. and Vishalakshi, G. J. and Paul, M. and Santhosh, M. S. and Shankar, R. L. and Sannaningaiah, Devaraja (2020) Protective Effect of Tamarind Seed Coat Ethanol Extract on Eryptosis Induced by Oxidative Stress. Biochemistry - Moscow, 85 (1). pp. 119-129.

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Suicidal erythrocyte death, or eryptosis, is the key event in eliciting anemia in numerous pathological conditions, including diabetes, chronic kidney disease, cancer, sepsis, etc. Oxidative stress is an important trigger in the acceleration of erythrocyte loss via eryptosis and an underlying mechanism of anemia emergence in the above pathologies. Therefore, there is an increasing demand for identification of antioxidants and anti-eryptotic agents forthe management of stress-related ailments. Here, we demonstrated the antioxidant and anti-eryptotic properties of the tamarind seed coat ethanol extract (TSCEE) against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress and eryptosis. The presence of probable secondary metabolites in the TSCEE extract was investigated by RP-HPLC. Active groups present in the TSCEE were studied by the Fourier-transform infrared spectroscopy. Cyclic voltammetric studies confirmed the antioxidant potential of TSCEE. The protective effect of TSCEE on red blood cells was confirmed by assessing various eryptotic markers, such as reactive oxygen species generation, intracellular calcium levels, and phosphatidylserine exposure. TSCEE reduced lipid peroxidation and protein carbonyl content and restored the levels of glutathione, antioxidant enzymes, and enzymes involved in glutathione replenishment. In conclusion, TSCEE was found to exhibit multiple therapeutic properties, which makes it a. promising agent for treating oxidative stress-induced eryptosis and subsequent anemia in various pathologies.

Item Type: Article
Uncontrolled Keywords: tamarind seed coat ethanol extract; reactive oxygen species; eryptosis; glutathione; glucose-6-phosphate dehydrogenase; antioxidants; 2,2 `-azobis(2-amidinopropane) dihydrochloride
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Mr Umendra uom
Date Deposited: 02 Feb 2021 07:07
Last Modified: 02 Feb 2021 07:07

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