Potential Antileptospiral Constituents from Phyllanthus amarus

Chandan, S. and Umesha, S. and Prasad, Shiva K. and Balamurugan, V. and Chandrashekar, S. and Kumar, Santosh S. R. and Ramu, Ramith and Shirahatti, Prithvi S. and Syed, Asad and Elgorban, Abdallah M. (2020) Potential Antileptospiral Constituents from Phyllanthus amarus. Pharmacognosy Magazine, 16 (70). S371-S378. ISSN 0976-4062

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Abstract

Background: Phyllanthus amarus (PA) is a well-known herb for its medicinal properties and widely used worldwide. PA has a significant role in Indian Ayurveda system of medicine for treating various ailments such as gonorrhea, menorrhagia, and other genital infections. Objectives: The aim of the study is to investigate antileptospiral activity and isolate the potential antileptospiral constituents of the methanol extract of PA (MPA). Materials and Methods: The primary pharmacological tests for leptospirosis were investigated by test tube dilution technique and microdilution technique. To examine the morphogenesis of experimental leptospirosis by morphologic and histological methods, albino mice were inoculated intraperitoneally with Leptospira interrogans sero group Icterohaemorrhagiae strains. Results: The activity-guided repeated fractionation for MPA through silica gel column chromatography yielded three compounds that exhibited antioxidant and in vitro, in vivo, and in silico antileptospiral activities. Based on diverse physicochemical and spectroscopic analyses (viz., 13C NMR,1H NMR, ultraviolet UV], IR, and mass spectroscopy), the potential constituents were elucidated as 5-(3-(3,4-dimethoxybenzyl)-4-methoxy-2-(methoxymethyl)butyl)4,7- dimethoxybenzod] 1,3] dioxole(C1), 1-(3-( 3,4- dimethoxybenzyl)- 4-methoxy- 2-(methoxymethyl) butyl)- 2,3,4,5tetramethoxybenzene(C2), and 4-(3-(3,4dimethoxybenzyl)-4-methoxy-2-(methoxymethyl)butyl)-3,6-dimethox ybenzene-1,2-diol (C3). The histopathological examinations of both kidney and liver showed promising activity with C3 at 75 and 100 mu g/mL, respectively. Conclusion: The in vitro, in vivo, and in silico studies revealed that benzo methoxy class of compounds has great potential as antileptospiral agents.

Item Type:	Article
Uncontrolled Keywords:	Antileptospiral activity; antioxidant; bioactive compounds; in silico; Leptospira; Phyllanthus amarus
Subjects:	B Life Science > Biotechnology
Divisions:	Department of > Biotechnology
Depositing User:	Mr Umendra uom
Date Deposited:	16 Feb 2021 09:23
Last Modified:	27 May 2022 05:17
URI:	http://eprints.uni-mysore.ac.in/id/eprint/15600

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