Characterization of ankaflavin from Penicillium aculeatum and its cytotoxic properties

Krishnamurthy, Soumya and Narasimha Murthy, Konappa and Thirumale, Sharmila (2020) Characterization of ankaflavin from Penicillium aculeatum and its cytotoxic properties. Natural Product Research, 34 (11). pp. 1630-1635. ISSN 1478-6427

Text Characterization of ankaflavin from Penicillium.pdf - Published Version Restricted to Repository staff only Download (991kB) | Request a copy

Abstract

The pigment was extracted from Penicillium aculeatum, purified and characterized as Ankaflavin by spectroscopic analysis. The stability of the pigment was determined under various conditions and was found to possess high stability. The cytotoxicity property of the purified pigment was determined by MTT assay in MCF-7, HCT116 and PC-3 and the studies were compared with its activity in CHOK1 cells. In MCF-7 and in CHOK 1 cells, the pigment exhibited very less toxicity. However, significant cytotoxicity was observed in HCT116 and PC-3 cells with IC50 of 162 mu g mL(-1) and 85 mu g mL(-1) for HCT116 and PC-3 cells respectively. In vitro toxicity was tested by haemolysis assay and MTT assay in HEK 293 cells. The pigment showed least cytotoxicity (<5%) at 160 and 320 mu g mL(-1) concentrations HEK 293 cells and negligible (<5%) toxicity on human erythrocytes at 160 and 320 mu g mL(-1), the highest concentrations tested.

Item Type:	Article
Uncontrolled Keywords:	Ankaflavin; characterization; in vitro cytotoxicity; Penicillium aculeatum
Subjects:	B Life Science > Biotechnology
Divisions:	Department of > Biotechnology
Depositing User:	Mr Umendra uom
Date Deposited:	20 Feb 2021 06:13
Last Modified:	20 Feb 2021 06:13
URI:	http://eprints.uni-mysore.ac.in/id/eprint/15568

Actions (login required)

View Item