Tumoricidal and Bactericidal Properties of ZnONPs Synthesized UsingCassia auriculataLeaf Extract

Shiva Prasad, K. and Prasad, Shashanka K. and Ansari, Mohammad Azam and Alzohairy, Mohammad A. and Alomary, Mohammad N. and AlYahya, Sami and Srinivasa, Chandrashekar and Murali, Mahadevamurthy and Veena, M. A. and Chandan, S. (2020) Tumoricidal and Bactericidal Properties of ZnONPs Synthesized UsingCassia auriculataLeaf Extract. biomolecules, 10 (7). ISSN 2218-273X

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Abstract

In this work, we aimed to synthesize zinc oxide nanoparticles (ZnONPs) using an aqueous extract ofCassia auriculataleaves (CAE) at room temperature without the provision of additional surfactants or capping agents. The formation of as-obtained ZnONPs was analyzed by UV-visible (ultraviolet) absorption and emission spectroscopy, X-ray photoemission spectroscopy (XPS), X-ray diffraction analysis (XRD), energy dispersive X-ray diffraction (EDX), thermogravimetric analysis/differential thermal analysis (TGA-DTA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and selected area electron diffraction (SAED). The XRD results reflect the wurtzite structure of as-prepared ZnONPs, which produced diffraction patterns showing hexagonal phases. The SEM images indicate that the morphology of as-prepared ZnONPs is composed of hexagonal nanostructures with an average diameter of 20 nm. The HR-TEM result shows that the inter-planar distance between two lattice fringes is 0.260 nm, which coincides with the distance between the adjacent (d-spacing) of the (002) lattice plane of ZnO. The fluorescence emission spectrum of ZnONPs dispersed in ethanol shows an emission maximum at 569 nm, revealing the semiconductor nature of ZnO. As-obtained ZnONPs enhanced the tumoricidal property of CAE in MCF-7 breast cancer cells without significant inhibition of normal human breast cells, MCF-12A. Furthermore, we have studied the antibacterial effects of ZnONPs, which showed direct cell surface contact, resulting in the disturbance of bacterial cell integrity.

Item Type:	Article
Uncontrolled Keywords:	zinc oxide nanoparticles (ZnONPs); Cassia auriculata; anticancer property; X-ray photoemission spectroscopy (XRD) pattern
Subjects:	B Life Science > Botany
Divisions:	Department of > Botany
Depositing User:	Mr Umendra uom
Date Deposited:	01 Apr 2021 10:23
Last Modified:	24 Nov 2022 06:03
URI:	http://eprints.uni-mysore.ac.in/id/eprint/15555

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