Mycosynthesis of ZnO Nanoparticles UsingTrichodermaspp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect againstXanthomonas oryzaepv.oryzae

Shobha, Balagangadharaswamy and Lakshmeesha, Thimappa Ramachandrappa and Ansari, Mohammad Azam and Almatroudi, Ahmad and Alzohairy, Mohammad A. and Basavaraju, Sumanth and Alurappa, Ramesha and Niranjana, Siddapura Ramachandrappa and Chowdappa, Srinivas (2020) Mycosynthesis of ZnO Nanoparticles UsingTrichodermaspp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect againstXanthomonas oryzaepv.oryzae. Journal of Fungi, 6 (3). ISSN 2309-608X

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Abstract

The Plant Growth Promoting Fungi (PGPF) is used as a source of biofertilizers due to their production of secondary metabolites and beneficial effects on plants. The present work is focused on the co-cultivation ofTrichodermaspp. (T. harzianum(PGT4),T. reesei(PGT5) andT. reesei(PGT13)) and the production of secondary metabolites from mono and co-culture and mycosynthesis of zinc oxide nanoparticles (ZnO NPs), which were characterized by a UV visible spectrophotometer, Powder X-ray Diffraction (PXRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDAX) and Transmission Electron Microscope (TEM) and Selected Area (Electron) Diffraction (SAED) patterns. The fungal secondary metabolite crude was extracted from the mono and co-culture ofTrichodermaspp. And were analyzed by GC-MS, which was further subjected for antibacterial activity againstXanthomonas oryzaepv.Oryzae, the causative organism for Bacterial Leaf Blight (BLB) in rice. Our results showed that the maximum zone of inhibition was recorded from the co-culture ofTrichodermaspp. rather than mono cultures, which indicates that co-cultivation of beneficial fungi can stimulate the synthesis of novel secondary metabolites better than in monocultures. ZnO NPs were synthesized from fungal secondary metabolites of mono cultures of Trichoderma harzianum (PGT4), Trichoderma reesei (PGT5), Trichoderma reesei (PGT13) and co-culture (PGT4 + PGT5 + PGT13). These ZnO NPs were checked for antibacterial activity against Xoo, which was found to be of a dose-dependent manner. In summary, the biosynthesized ZnO NPs and secondary metabolites from co-culture ofTrichodermaspp. are ecofriendly and can be used as an alternative for chemical fertilizers in agriculture.

Item Type: Article
Uncontrolled Keywords: fungal nanotechnology; Trichodermaspp; ZnO nanoparticles; antibacterial activityXanthomonas oryzaepv; oryzae; co-cultivation; secondary metabolites
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Mr Umendra uom
Date Deposited: 05 Mar 2021 06:25
Last Modified: 05 Mar 2021 06:25
URI: http://eprints.uni-mysore.ac.in/id/eprint/15523

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