Biosynthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Passiflora subpeltata: Characterization and Antibacterial Activity Against Escherichia coli Isolated from Poultry Faeces

Chunchegowda, Udayashankar Arakere and Shivaram, Ashwini Bagepalli and Mahadevamurthy, Murali and Ramachndrappa, Lakshmeesha Thimappa and Lalitha, Sreelatha Gopalakrishna and Krishnappa, Hemanth Kumar Naguvanahalli and Anandan, Satish and Sudarshana, Brijesh Singh and Chanappa, Eswaraiah Ganadhal and Ramachandrappa, Niranjana Siddapura (2020) Biosynthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Passiflora subpeltata: Characterization and Antibacterial Activity Against Escherichia coli Isolated from Poultry Faeces. Journal of Cluster Science. ISSN 1572-8862

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Abstract

The current study was undertaken to investigate the antibacterial (against molecular characterized E. coli isolated from poultry faeces) potential of biosynthesized zinc oxide nanoparticles (ZnO-NPs) from Passiflora subpeltata Ortega aqueous leaf extract. The biosynthesized nanoparticles were subjected to physico-chemical characterization to study shape, size and purity by UV-Vis spectroscopy, X-Ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and Transmission Electron Microscopy (TEM). The molecular identification of isolated E. coli from faeces samples was carried out by using 16-23s rRNA primers. The results of the physico-chemical characterization revealed that the biosynthesized nanoparticles were of 93.7% purity with an average size between 45 and 50 nm. The ZnO-NPs offered significant inhibition against the isolated Gram-negative E. coli with MIC at 62.5 mu g mL(-1) concentration. The antibacterial potential of ZnO NPs against E. coli has also been investigated by the cell viability test, and further the effects of ZnO NPs on bacterial morphological structures was analysed by SEM and TEM.

Item Type: Article
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Mr Umendra uom
Date Deposited: 30 Mar 2021 09:46
Last Modified: 30 Mar 2021 09:46
URI: http://eprints.uni-mysore.ac.in/id/eprint/15479

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