Drupin, a cysteine protease from Ficus drupacea latex accelerates excision wound healing in mice

Manjuprasanna, Vaddarahally N. and Rudresha, Gotravalli and Urs, Amog P. and Gowda, Mallanayakanakatte D. Milan and Rajaiah, Rajesh and Vishwanath, Bannikuppe S. (2020) Drupin, a cysteine protease from Ficus drupacea latex accelerates excision wound healing in mice. International Journal of Biological Macromolecules, 165 (A). pp. 691-700. ISSN 1879-0003

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Abstract

Wound healing is a tightly regulated physiological process that restores tissue integrity after injury. Plant latex proteases (PLPs) are considered an integral part in herbal wound care as it interferes at different phases of the wound healing process. Although many studies have reported the involvement of PLPs in healing process, an in-depth investigation is required to understand the molecular mechanism. Hence, the effect of PLPs with fibrinolytic activity on wound healing was investigated systematically using mouse excision wound model. Among 29 latices from Ficus genus tested, Ficus drupacea exhibited potent fibrinolytic activity. Cysteine protease responsible for fibrinolysis was purified from the F. drupacea latex named it as drupin, tested for its wound healing efficacy. The accelerated wound healing was mediated by downregulation of matrix metalloprotease (MMP)-9 without altering MMP-8 expression. Besides, drupin enhanced the rate of collagen synthesis at the wound site by increasing arginase 1 activity. And also, drupin increased the expression of arginase 1 inmacrophages and involved in cell proliferation, and migration via MAP kinase and PI3K/Akt pathways. Overall, the present study highlights the interference of drupin in wound healing by increased arginase 1 activity and collagen synthesis, and cell proliferation and migration. (C) 2020 Elsevier B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Wound healing; Fibrinolysis; MMPs
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Mr Umendra uom
Date Deposited: 01 Apr 2021 10:21
Last Modified: 01 Apr 2021 10:21
URI: http://eprints.uni-mysore.ac.in/id/eprint/15456

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