Detection, using antibodies, of pharmacologically active-sites, apart from the catalytic site, on venom phospholipase-a2

Kasturi, S and Veerabasappa Gowda, T. (1990) Detection, using antibodies, of pharmacologically active-sites, apart from the catalytic site, on venom phospholipase-a2. Toxicon, 28 (1). pp. 92-99.

Text savedrecs(86).bib Download (994B)

Abstract

Detection, using antibodies, of pharmacologically active sites, apart from the catalytic site, on venom phospholipase A2. Toxicon28, 91–99, 1990.—Polyclonal antibodies to a purified neurotoxic phospholipase A2 VRV PL-V from Vipera russelli venom were raised in rabbits. The gamma globulin fraction from the serum of the rabbits injected with VRV PL-V-toxoid (anti VRV PL-V antibodies) is termed anti PL-V Ig. Anti PL-V Ig neutralized the neurotoxic symptoms and lethal effects of neurotoxic PLA2s (VRV PL-V, VRV PL-VI and VRV PL-VIIIa) and neurotoxic symptoms of whole V. russelli venom, without affecting their phospholipase A2 activities. Their edema inducing activity was also unaffected. Anti VRV PL-V-antibodies inhibited the anticoagulant potencies of the neurotoxic PLA2s (VRV PL-V, VRV PL-VI and VRV PL-VIIIa) from V. russelli venom and VRV PL-V-toxoid. The myonecrotic activity of VRV PL-V, VRV PL-VIIIa and whole venom remain unaffected in presence of anti PL-V Ig. The hemoglobinuria induced in rabbits by injection of VRV PL-V-toxoid was inhibited by anti VRV PL-V antibodies. These results indicate the presence of multiple pharmacological sites apart from the catalytic site on V. russelli PLA2 molecules.

Item Type:	Article
Uncontrolled Keywords:	Pharmacology & Pharmacy; Toxicology
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Dr Raju C
Date Deposited:	21 Jan 2021 09:41
Last Modified:	12 Jul 2022 06:51
URI:	http://eprints.uni-mysore.ac.in/id/eprint/15437

Actions (login required)

View Item