Capsaicin inhibits calmodulin-mediated oxidative burst in rat macrophages

Savitha, G. and Shoba Panchanathan and Bharathi P. Salimath (1990) Capsaicin inhibits calmodulin-mediated oxidative burst in rat macrophages. ScienceDirect, 2 (6). pp. 577-585. ISSN 0898-6568

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In this study we seek to elucidate the interaction of capsaicin with the calmodulin mediated signal pathways in macrophages, by comparing its action on macrophage functions with a known calmodulin antagonist, fluphenazine. Kinetics of capsaicin uptake by macrophages (10(3) cells) revealed that a maximum of 200-mu-M capsaicin was taken up with 10 min. Ca2+ ionophore triggered generation of superoxide anion and hydrogen peroxide by macrophages was inhibited in a dose-dependent manner by fluphenazine (IC50, 20-mu-M and 12-mu-M, respectively) and also by capsaicin (IC50, 30-mu-M and 9-mu-M, respectively), suggesting an involvement of calmodulin in the regulation of NADPH oxidase. In vitro both fluphenazine and capsaicin inhibited Ca2+-Mg2+ ATPase and cAMP-phosphodiesterase from macrophages and this inhibition was reversed by exogenous addition of calmodulin. Fluorescence studies revealed a direct Ca2+ dependent interaction of capsaicin with calmodulin. From these results we suggest that capsaicin acts via calmodulin to inhibit stimulus-induced macrophage oxidative burst and also that calmodulin regulates the oxidative burst in macrophages.

Item Type: Article
Uncontrolled Keywords: Macrophages; Oxidative Burst; Calmodulin; Capsaicin; Signal Transduction
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Dr Raju C
Date Deposited: 21 Jan 2021 09:45
Last Modified: 20 Jun 2022 11:27

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