Studies On Induction of Resistance Against Nuclear Polyhedrosis in Silkworm Bombyx Mori L And Its Biochemical Aspects

Mahesha, H. B. and Thejaswini, P. H. (2009) Studies On Induction of Resistance Against Nuclear Polyhedrosis in Silkworm Bombyx Mori L And Its Biochemical Aspects. In: National Conference on Recent Trends In Animal Physiology, 29-30 October 2009, Organized by University of Mysore, Mysore.

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Abstract

Induction of resistance is a mechanism which is operative in nature by the nature itself. It is vaccination protocol, which involves exposing the susceptible host to the pathogen at low, insufficient dose to cause disease or by using abiotic and biotic components which is generalized by the term inducers. To induce resistance in silkworm against nuclear polyhedrosis virus (NPV), two sets of experiments were carried out. For both the experiments five silkworm races i.e., NB4D2, CSR2 (Bivoltines), Pure Mysore, C. nichi (Multivoltines) and a hybrid (PM X CSR2) were used. In the first set of experiments silkworms were fed with known amount of inactivated nuclear polyhedral inclusion bodies (PIBs) thrice at various time intervals for multiple immunization. In the second set of experiments such a type of treatment was given only once. At the age of fifth instar second day, the silkworms of all the batches i.e., control, single immunized and multiple immunized batches were challenge inoculated with known amount of live PIBs. Among immunization methods, the multiple immunization method was more effective when compared to single immunization method, as all the races indicated by showing high survival rate. Also variation in qualitative and quantitative biochemical composition was observed with respect to haemolymph proteins and enzymes such as amylase, succinate dehydrogenase, superoxide dismutase and beta esterase when compared with the unimmunized controls. The results pave light towards use of immunization method to induce resistance in silkworms against the dreaded NPV. The technique can be further extended to other diseases also.

Item Type: Conference or Workshop Item (Paper)
Subjects: Agriculture Biological Sciences > Biotechnology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biotechnology
Depositing User: Swamy D
Date Deposited: 08 Aug 2013 08:01
Last Modified: 07 Dec 2015 11:14
URI: http://eprints.uni-mysore.ac.in/id/eprint/14624

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