A Low Molecular Weight Isoform of Hyaluronidase: Purification from Indian Cobra (Naja naja) Venom and Partial Characterization

Girish, K. S. and Kemparaju, K. (2005) A Low Molecular Weight Isoform of Hyaluronidase: Purification from Indian Cobra (Naja naja) Venom and Partial Characterization. Biochemistry (Moscow), 70 (6). pp. 708-712. ISSN 0006-2979

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Official URL: http://dx.doi.org/10.1007/s10541-005-0172-6

Abstract

A low molecular weight isoform of hyaluronidase (NNH2) has been isolated from Indian cobra (Naja naja) venom by successive chromatography on Sephadex G-75 and CM-Sephadex C-25 columns. The apparent molecular weight determined by SDS-PAGE is 52 kD, and the pI value is 9.7. NNH2 is an endoglycosidase and exhibits in vitro absolute specificity for hyaluronan; it also hydrolyzed hyaluronan in human skin sections. NNH2 is nontoxic, but it indirectly potentiates the hemorrhagic activity of hemorrhagic complex-I. Curcumin, indomethacin, and tannic acid inhibited dose dependently the degradation of hyaluronan by NNH2

Item Type: Article
Additional Information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Biochemistry (Moscow) [Field not mapped to EPrints]
Uncontrolled Keywords: hyaluronidase, hyaluronan, spreading factor, Naja naja venom
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Depositing User: Manjula P
Date Deposited: 06 Aug 2013 10:39
Last Modified: 06 Aug 2013 10:39
URI: http://eprints.uni-mysore.ac.in/id/eprint/14589

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