Synthesis of shorter active analogues of bactenecin7: The effect of change of N-terminal configuration on antimicrobial activity

Abiraj, K. and Prakasha Gowda, A. S. and Channe Gowda, D. (2002) Synthesis of shorter active analogues of bactenecin7: The effect of change of N-terminal configuration on antimicrobial activity. Letters in Peptide Science, 9 (6). pp. 283-290.

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Abstract

Bactenecin7, a cationic antibacterial peptide, contains a repeating region of Xaa-Pro-Arg-Pro (Xaa=hydrophobic residues). A series of peptides, Xaa-Pro-Arg-Pro (Xaa=D-Ala, D-Leu, D-Val, D-Phe and D-Lys) were synthesized to investigate the effect of change ofN-terminal configuration on antimicrobial activity. The conformational preferences of these peptides in water and TFE were examined by circular dichroism. All the synthetic peptides with D-amino acid substitution atN-terminal showed potent antifungal activity againstAspergillus niger, Aspergillus flavus andFusarium moniliforme at the concentration level of 8–10 μg ml−1. But the same tetrapeptides were unable to kill or suppress the growth of gram-negative and gram-positive bacteria such asEscherichia coli HB101,Pseudomonas aeruginosa, Klebsiella pneumoniae andStaphylococcus aureus even at the concentration level of 400 μg ml−1. The present study reveals that the change of configuration at theN-terminal of tetrapeptide has negative impact on antibacterial activity but enhanced antifungal activity.

Item Type:	Article
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	Manjula P Library Assistant
Date Deposited:	22 Dec 2020 07:45
Last Modified:	04 Jul 2022 05:10
URI:	http://eprints.uni-mysore.ac.in/id/eprint/14274

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