A platelet aggregation inhibitor phospholipase a2 from russell's viper (vipera russelli) venom: Isolation and characterization

Prasad, B. N. and Kemparaju, K. and Bhatt, K. G. S. and Gowda, T. Veerabasappa (1996) A platelet aggregation inhibitor phospholipase a2 from russell's viper (vipera russelli) venom: Isolation and characterization. Toxicon , 34 (10). pp. 1173-1185. ISSN 0041-0101

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Official URL: http://dx.doi.org/10.1016/0041-0101(96)00033-5

Abstract

Vipera russelli venom is reported to contain multiple forms of phospholipase A2 (PLA2). Three PLA2s (VRV-PL-V, VRV-PL-VI and VRV-PL-Villa) have been purified and characterized in this laboratory. A PLA2 (VRV-PL-IIIb) inhibiting platelet aggregation was purified from the same venom in two steps involving CM-Sephadex C-25 column chromatography followed by gel filtration on Sephadex G-50. VRV-PL-IIIb is a basic (pI 7.3–7.7) isoenzyme, with a mol. wt between 14,000 and 15,000. It induced neurotoxic symptoms in experimental mice with an i.p. ld50 of 5.2 mg/kg body weight. VRV-PL-IIIb inhibits ADP-induced platelet aggregation in a dose-dependent manner. It induced oedema in the foot pads of mice and is devoid of anticoagulant, myotoxic and direct haemolytic activities. Antibodies to VRV-PL-IIIb showed a single precipitin line against the antigen as well as whole venom.

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 1996/10// [EPrints field already has value set] JO - Toxicon [Field not mapped to EPrints]
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Biochemistry
Depositing User: Ambika P Patil
Date Deposited: 22 Jul 2013 06:44
Last Modified: 11 Sep 2013 06:04
URI: http://eprints.uni-mysore.ac.in/id/eprint/14012

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