Culturing Sclerospora sorghi in callus tissue of sorghum

Kaveriappa, K. M. and Safeeulla, K. M. and Shaw, C. G. (1980) Culturing Sclerospora sorghi in callus tissue of sorghum. Proceedings: Plant Sciences, 89 (2). pp. 131-138. ISSN 03700-097

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Sclerospora sorghi has been cultured in callus tissues of Sorghum vulgare maintained on a modified White's medium. Cultures of healthy and diseased calluses were initiated from surface sterilised explants of stem apices and axes of inflorescences of healthy and diseased plants, and subcultured. The fungus developed internally in the callus and also spread over its surface as a white cottony mycelium. Healthy callus tissue and the sorghum seedlings were infected by placing them in contact with the mycelium produced on diseased callus. Mycelium spread from infected callus onto the culture medium, but remained viable only if physical contact with the callus was maintained. Only mycelium and oogonia were observed in infected callus. The number of nuclei in the oogonia varied. Some oogonia were abnormal both in size and shape. None of the oogonia developed oospores. © 1980 Indian Academy of Sciences.

Item Type: Article
Depositing User: Dhruva Kumar
Date Deposited: 12 May 2021 16:03
Last Modified: 12 May 2021 16:03

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