Thimme Gowda, A. and Made Gowda, N. M. and Harsh Gowda, S. and Rancappa, K. S. (1985) Application of Azure C for the extractive spectrophotometric determination of microgram amounts of penicillin. Journal of Pharmacological Methods, 13 (3). pp. 275-280. ISSN 0160-5402
Full text not available from this repository. (Request a copy)Abstract
A simple, accurate, and rapid method for the quantitative determination of penicillin is proposed. The method is based on the formation of a blue penicillin-Azure-C ion-pair that can be extracted into chloroform in phosphate-citric acid buffer. The molar absorptivities for sodium penicillin G and potassium penicillin V at 635 nm were 5.46 � 103 and 2.19 � 104 l/mol/cm, respectively. Beer's law was valid over the concentration range of 4-80 μg ml for sodium penicillin G and 3-55 μg ml for potassium penicillin V. Maximum absorbance was obtained almost instantaneously and was stable for several days. The method was successfully applied to pharmaceutical preparations. © 1985.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | methodology, nonhuman, Spectrophotometry, priority journal, drug determination, spectrophotometry, Hydrogen-Ion Concentration, drug analysis, azure c, Azure Stains, Buffers, Indicators and Reagents, penicillin g, Penicillin G, penicillin v, Penicillin V, Penicillins, Phenothiazines |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Chemistry |
| Depositing User: | Dhruva Kumar |
| Date Deposited: | 06 May 2021 10:35 |
| Last Modified: | 16 Jul 2022 06:27 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/13678 |
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