Aginoside saponin, a potent antifungal compound, and secondary metabolite analyses from Allium nigrum L.

Mostafa, A. and Sudisha, J. and El-Sayed, M. and Ito, Shin-ichi and Ikeda, T. and Yamauchi, N. and Shigyo, M. (2013) Aginoside saponin, a potent antifungal compound, and secondary metabolite analyses from Allium nigrum L. PHYTOCHEMISTRY LETTERS, 6 (2). pp. 274-280.

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Official URL: http://dx.doi.org/10.1016/j.phytol.2013.03.001

Abstract

The HPLC and spectral analyses of cysteine sulfoxides (CSOs), total polyphenols (TP), and total saponins revealed quantitative variations within the different organs of Allium nigrum L. A large accumulation of CSOs was detected in the bulb (0.367 mg/g fw), of TP in the leaf (116.05 mg CE/100 g fw), and of saponins in the root (19.38 mg/g dw). Phytochemical and chromatographical investigations of A. nigrum root extract led to the isolation of a spirostane-type glycoside or aginoside. The structure was elucidated by spectroscopic analysis (2D NMR, FABMS, HR-ESI-MS). The structure of the aginoside was identified as 25(R,S)-5 alpha-spirostan-2 alpha,3 beta,6 beta-trio1-3-O-beta-D-glucopyranosyl-(1 -> 2)-O-beta-D-xylopyranosyl-(1 -> 3)]-O-beta-D-glucopyranosyl-(1 -> 4)-beta-D-galactopyranoside. The highest content of aginoside, 2.9 mg/g dw, was detected in the root. The in vitro and in vivo antifungal activity of aginoside was evaluated for the first time against phytopathogens. This compound showed significant (P < 0.05) antifungal activity depending on the concentration. (C) 2013 Phytochemical Society of Europe. Published by Elsevier B. V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Allium nigrum; Saponin; Aginoside; Antifungal activity; Cysteine sulfoxides; Polyphenols
Subjects: Agriculture Biological Sciences > Biotechnology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biotechnology
Divisions: PG Campuses > Manasagangotri, Mysore > Biotechnology
Depositing User: Venkatesha
Date Deposited: 10 Jul 2013 07:28
Last Modified: 06 Oct 2015 05:48
URI: http://eprints.uni-mysore.ac.in/id/eprint/13625

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