An approach to obtain specific polyclonal antisera to Xanthomonas campestris pv. cyamopsidis and its potential application in indexing of infected seeds of guar

Vijayanand, G. K. and Shylaja, M. D. and Krishnappa, M. and Shetty, H. Shekar (1999) An approach to obtain specific polyclonal antisera to Xanthomonas campestris pv. cyamopsidis and its potential application in indexing of infected seeds of guar. Journal of Applied Microbiology, 87 (5). pp. 711-717. ISSN 1364-5072

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Official URL: http://dx.doi.org/10.1046/j.1365-2672.1999.00914.x

Abstract

Clusterbean seed health testing is warranted since the pathogen (Xanthomonas campestris pv. cyamopsidis (Xccy)) is seed-borne and seed-transmitted. A polyclonal antibody was developed in rabbit via subcutaneous and intramuscular injections and characterized for sensitivity, specificity and its applicability to ELISA which: (i) was sensitive in detecting as few as 10<SUP>2</SUP> cells ml<SUP>?1</SUP> at a titre of 1:4000; (ii) was specific, since it reacted only with Xccy and not with other xanthomonads; (iii) reacted both with Xccy cells and culture filtrate, indicating that the antigenic determinant is a secretory component; (iv) was applicable and reliable in seed health testing since it reacted only with infected seeds and plant materials and not with healthy seeds and (v) a purified fraction of antibody was virulent-specific since heat-denatured and avirulent isolates were not detected. The ELISA thus developed is highly reproducible and therefore suitable for the evaluation of the potential disease status of seeds and plant health, which is appropriate for routine seed health testing.

Item Type: Article
Additional Information: Unmapped bibliographic data: Y1 - 1999/10// [EPrints field already has value set] AV - public [Field not mapped to EPrints]
Subjects: Agriculture Biological Sciences > Biotechnology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biotechnology
Divisions: PG Campuses > Manasagangotri, Mysore > Biotechnology
Depositing User: Ambika P Patil
Date Deposited: 15 Jul 2013 06:37
Last Modified: 13 Aug 2013 06:22
URI: http://eprints.uni-mysore.ac.in/id/eprint/13471

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