Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes

Harish, S. K. and Guruprasad, K. P. and Mahmood, Riaz and Vasudev, V. and Manjunath, K. R. and Chethan, G. K. (1998) Adaptive response to low dose of EMS or MMS in human peripheral blood lymphocytes. Indian Journal of Experimental Biology, 36 (11). pp. 1147-1150.

Full text not available from this repository. (Request a copy)


Human peripheral blood lymphocytes stimulated in vitro for 6 hr were exposed to a low (conditioning) dose of ethyl methanesulfonate (EMS; 1.5 x 10(-4) M) or methyl methanesulfonate (MMS; 1.5 x 10(-5) M). After 6 hr, the cells were treated with a high (challenging) concentration of the same agent (1.5 x 10(-3) M EMS or 1.5 x 10(-4) M MMS). The cells that received both conditioning and challenging doses became less sensitive to the induction of sister chromatid exchanges (SCEs) than those which did not receive the pretreatment with EMS or MMS. They responded with lower frequencies of SCEs. This suggests that conditioning dose of EMS or MMS has offered the lymphocytes to have decreased SCEs. This led to the realization that pre-exposure of lymphocytes to low dose can cause the induction of repair activity. This is a clear indication of the existence of adaptive response induced by alkylating agents whether it is ethylating or methylating in human lymphocytes in vitro.

Item Type: Article
Additional Information: Publisher: National Institute of Science Communication, CSIR
Uncontrolled Keywords: adaptive response human lymphocyte EMS MMS, sister chromatid exchange alkylating agent
Subjects: B Life Science > Zoology
Divisions: Department of > Zoology
Depositing User: Dhruva Kumar
Date Deposited: 19 Dec 2020 10:36
Last Modified: 19 Dec 2020 10:36

Actions (login required)

View Item View Item