Use of chloramine-T and two dyes in the sensitive determination of stavudine in pharmaceuticals.

Basavaiah, K. and Ramakrishna, V. and Anil Kumar, U. R. (2007) Use of chloramine-T and two dyes in the sensitive determination of stavudine in pharmaceuticals. Revista Brasileira de Ciencias Farmaceuticas, 43 (3, Cop). pp. 471-479. ISSN 1516-9332

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Official URL: http://dx.doi.org/10.1590/S1516-93322007000300016

Abstract

Three new methods are described for the assay of stavudine (STV) in bulk drug and in dosage forms using chloramine-T (CAT) and 2 dyes, methyl orange and indigocarmine, as reagents. Titrimetry involves treating STV with a measured excess of CAT in hydrochloric acid medium, and after the oxidn. of STV is judged to be complete, the unreacted oxidant is detd. iodometrically. Spectrophotometric methods entail the addn. of a known excess of CAT to STV in hydrochloric acid medium followed by detn. of residual oxidant by reacting with a fixed amt. of either methyl orange and measuring the absorbance at 520 nm (Method A) or indigo carmine and measuring the absorbance at 610 nm (Method B). In all the methods, the amt. of CAT reacted corresponds to the amt. of STV. In titrimetric method, the reaction follows 1:1 stoichiometry (STV: CAT), and is applicable over the range 1.5-10 mg of STV. In spectrophotometric methods, the absorbance is found to increase linearly with concn. of STV. The systems obey Beer's law for 0.2-2.0 and 1.0-10.0 μg/mL for method A and method B, resp. The apparent molar absorptivities are calcd. to be 5.7×104 and 1.5×104 L/mol/cm for method A and method B, resp., and the corresponding Sandell sensitivity values are 0.004 and 0.015 μg/cm2. The limits of detection and quantification are reported for both methods. Intra-day and inter-day precision and accuracy of the developed methods were evaluated as per the current ICH guidelines. The methods were successfully applied to the assay of STV in tablet and capsule formulations and the results were compared with those of a ref. method by applying Student's t-test and F-test. No interference was obsd. from common tablet adjuvants. The accuracy and reliability of the methods were further ascertained by performing recovery expts. via std.-addn. method. [on SciFinder(R)]

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2007/// [EPrints field already has value set] M3 - 10.1590/S1516-93322007000300016 [Field not mapped to EPrints] JA - Rev. Bras. Cienc. Farm. [Field not mapped to EPrints]
Uncontrolled Keywords: stavudine detn titrimetry spectrophotometry chloramineT dye
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Dhruvakumar
Date Deposited: 11 Jun 2013 11:15
Last Modified: 16 Aug 2013 06:22
URI: http://eprints.uni-mysore.ac.in/id/eprint/12388

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