Use of ceric ammonium sulphate and two dyes, methyl orange and indigo carmine, in the determination of lansoprazole in pharmaceuticals.

Basavaiah, K. and Ramakrishna, V. and Anil Kumar, U. R. (2007) Use of ceric ammonium sulphate and two dyes, methyl orange and indigo carmine, in the determination of lansoprazole in pharmaceuticals. Acta Pharmaceutica (Zagreb, Croatia), 57 (2, Cop). pp. 211-220. ISSN 1330-0075

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Abstract

Two spectrophotometric methods are proposed for the assay of lansoprazole (LPZ) in bulk drug and in dosage forms using ceric ammonium sulfate (CAS) and 2 dyes, methyl orange and indigo carmine, as reagents. The methods involve addn. of a known excess of CAS to LPZ in acid medium, followed by detn. of residual CAS by reacting with a fixed amt. of either methyl orange, measuring the absorbance at 520 nm (method A), or indigo carmine, measuring the absorbance at 610 nm (method B). In both methods, the amt. of CAS reacted corresponds to the amt. of LPZ and the measured absorbance was found to increase linearly with the concn. of LPZ, which is corroborated by the correlation coeffs. of 0.9979 and 0.9954 for methods A and B, resp. The systems obey Beer's law for 0.5-7.0 μg mL-1 and 0.25-3.0 μg mL-1 for methods A and B, resp. The apparent molar absorptivities were calcd. to be 3.0 × 104 and 4.4 × 104 L mol-1 cm-1 for methods A and B, resp. The limits of detection (LOD) and quantification (LOQ) were calcd. to be 0.08 and 0.25 μg mL-1 for method A, and 0.09 and 0.27 μg mL-1 for method B, resp. The intra-day and inter-day precision and accuracy of the methods were evaluated according to the current ICH guidelines. Both methods were of comparable accuracy (er ≤ 2 %). Also, both methods are equally precise as shown by the relative std. deviation values < 1.5%. No interference was obsd. from common pharmaceutical adjuvants. The accuracy of the methods was further ascertained by performing recovery studies using the std. addn. method. The methods were successfully applied to the assay of LPZ in capsule prepns. and the results were statistically compared with those of the literature UV-spectrophotometric method by applying Student's t-test and F-test. [on SciFinder(R)]

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2007/// [EPrints field already has value set] M3 - 10.2478/v10007-007-0017-5 [Field not mapped to EPrints] JA - Acta Pharm. (Zagreb, Croatia) [Field not mapped to EPrints]
Uncontrolled Keywords: lansoprazole detn spectrophotometry ceric ammonium sulfate methyl orange, indigo carmine spectrophotometry lansoprazole detn ceric ammonium sulfate
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Dhruvakumar
Date Deposited: 11 Jun 2013 06:26
Last Modified: 23 Jul 2013 06:29
URI: http://eprints.uni-mysore.ac.in/id/eprint/12346

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