Dissection and Preparation of whole Mounts of Endosperm from the Seeds of Grevillea (Proteaceae)

Kausik, S. B. (1939) Dissection and Preparation of whole Mounts of Endosperm from the Seeds of Grevillea (Proteaceae). Stain Technology, 14 (2). pp. 43-46.

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Official URL: https://www.tandfonline.com/doi/abs/10.3109/105202...


It is sometimes very desirable to supplement the examination of serially cut microtome sections by dissection and study of whole mounts of objects. In the case of the seeds of Grerillea the endosperm forms a curious worm-like structure, the vermiform appendage, in elucidating the nature of which microtome sections alone are inadequate. The dissection of the endosperm for demonstrating this structure is very necessary; otherwise the structure is altogether overlooked. In dissecting out the endosperm, which is easily done, either fresh seeds or previously preserved seeds may be used; the former are easier for handling and yield more satisfactory results. In the case of fresh material, an immediate killing is necessary and any one of the ordinary killing fluids may be used; Bouin's fluid was tried by the writer and was entirely satisfactory. After killing, the material is stained and dehydrated, the several stages of which are best done on the slide alone on which the material is placed. Mounting is done in any of the mounting media, and Canada balsam answers the purpose quite well.

Item Type: Article
Additional Information: Place: United Kingdom Publisher: Taylor and Francis Ltd
Subjects: B Life Science > Botany
Divisions: Department of > Botany
Depositing User: Dhruva Kumar
Date Deposited: 07 May 2021 07:02
Last Modified: 07 May 2021 07:02
URI: http://eprints.uni-mysore.ac.in/id/eprint/12288

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