Detection of Phomopsis azadirachtae from dieback affected neem twigs, seeds, embryo by polymerase chain reaction.

Nagendra Prasad, M. N. and Shankara Bhat, S. and Charith Raj, A. P. and Janardhana, G. R. (2009) Detection of Phomopsis azadirachtae from dieback affected neem twigs, seeds, embryo by polymerase chain reaction. Archives of Phytopathology and Plant Protection, 42 (2, Cop). pp. 124-128. ISSN 0323-5408

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Official URL: http://dx.doi.org/10.1080/03235400600982584

Abstract

Twigs and seeds of Azadirachta indica (Neem) infected with dieback disease, collected from different regions of Karnataka, India were analyzed to det. the pathogens. Phomopsis azadirachtae, the causal agent of dieback disease of neem was found to be seed borne. Phomopsis azadirachtae was isolated on PDA and MEA from dieback-infected neem twigs, seeds and embryo. Phomopsis genus-specific primers (5.8S rDNA) were then used for the detection of Phomopsis azadirachtae, the causative agent of dieback of neem by polymerase chain reaction (PCR). Reactions were performed on DNA isolated from twigs, seeds and embryo of dieback-affected neem trees. Studies revealed the amplification of expected 141 bp DNA in Phomopsis azadirachtae isolated from various parts of diseased trees indicating the causal organism of dieback disease on neem. Isolation and identification by conventional technique takes around 15-21 days, whereas the present technique is capable of detecting very low propogules within 4-5 days. [on SciFinder(R)]

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2009/// [EPrints field already has value set] M3 - 10.1080/03235400600982584 [Field not mapped to EPrints] JA - Arch. Phytopathol. Plant Prot. [Field not mapped to EPrints]
Uncontrolled Keywords: Phomopsis detection neem Azadirachta rDNA specific PCR
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Kodandarama
Date Deposited: 10 Jun 2013 05:59
Last Modified: 30 Jul 2013 05:44
URI: http://eprints.uni-mysore.ac.in/id/eprint/12204

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