PCR-based detection of Phomopsis azadirachtae in die-back affected neem seeds.

Girish, K. and Shankara Bhat, S. and Raveesha, K. A. (2009) PCR-based detection of Phomopsis azadirachtae in die-back affected neem seeds. Archives of Phytopathology and Plant Protection, 42 (7, Cop). pp. 626-632. ISSN 0323-5408

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Official URL: http://dx.doi.org/10.1080/03235400701287628


Phomopsis azadirachtae Sateesh, Bhat & Devaki is the incitant of die-back disease of neem trees. Delayed appearance of conidia and presence of other microorganisms in the neem tissues are the obstacles in the rapid and accurate identification of P. azadirachtae. This work was carried out to develop a methodol. for rapid detection of the pathogen in diseased tissues esp. in the neem seeds. RDNA sequences of many Phomopsis spp. were retrieved from the database and were subjected for multiple alignment to select a 179 bp conserved sequence. This was used to design Phomopsis specific primer pair (forward and reverse) having the potential to produce a 154 bp product in PCR. The primer pair was utilized to detect the presence of P. azadirachtae in diseased neem seeds and other tissues. This is the first report on the PCR-based detection of P. azadirachtae directly in die-back diseased neem tissues. This method can be employed for rapid and reliable detection of P. azadirachtae in die-back affected neem seeds. Hence it will have very good application in seed health testing labs. [on SciFinder(R)]

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2009/// [EPrints field already has value set] M3 - 10.1080/03235400701287628 [Field not mapped to EPrints] JA - Arch. Phytopathol. Plant Prot. [Field not mapped to EPrints]
Uncontrolled Keywords: sequence polymerase chain reaction dieback seed Phomopsis
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Kodandarama
Date Deposited: 07 Jun 2013 07:36
Last Modified: 30 Jul 2013 05:42
URI: http://eprints.uni-mysore.ac.in/id/eprint/12152

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