Simple spectrophotometric methods for the determination of zidovudine in pharmaceuticals using chloramine-T methylene blue and rhodamine-B as reagents.

Basavaiah, K. and Anil Kumar, U. R. (2006) Simple spectrophotometric methods for the determination of zidovudine in pharmaceuticals using chloramine-T methylene blue and rhodamine-B as reagents. E-Journal of Chemistry, 3 (12, Co). pp. 173-181.

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Official URL: http://dx.doi.org/10.1155/2006/248765

Abstract

Two new spectrophotometric methods are proposed for the detn. of zidovudine(ZDV) in pharmaceuticals. The methods use chloramine-T (CAT) and two dyes, methylene blue and rhodamine-B, as reagents and are based on adding of a known excess of CAT to ZDV in hydrochloric acid medium followed by detn. of residual oxidant by reacting with a fixed amt. of either methylene blue and measuring the absorbance at 665 nm (Method A) or rhodamine B and measuring the absorbance at 555 nm (Method B). In both methods, the amt. of CAT reacted corresponds to the amt. of ZDV. The absorbance measured is found to increase linearly with concn. of ZDV. Under the optimum conditions, ZDV could be assayed in the concn. range 1.25-15.0 and 0.25-3.0 μg mL-1 by method A and method B, resp. The apparent molar absorptivities are calcd. to be 7.7×103 and 5.6×104 L mol-1cm-1 for method A and method B, resp., and the corresponding Sandell sensitivity values are 0.035 and 0.005 μg cm-2. The limits of detection and quantification are reported for both methods. Intra-day and inter-day precision and accuracy of the developed methods were evaluated as per the current ICH guidelines. The proposed methods can be readily utilized for bulk drug and in pharmaceutical formulations. [on SciFinder(R)]

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2006/// [EPrints field already has value set] JA - E-J. Chem. [Field not mapped to EPrints]
Uncontrolled Keywords: zidovudine detn spectroscopy chloramine T rhodamine B
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Dhruvakumar
Date Deposited: 31 May 2013 06:05
Last Modified: 23 Jul 2013 06:46
URI: http://eprints.uni-mysore.ac.in/id/eprint/11913

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