Syed, Baker and Nalini Bisht and Bhat, Prithvi S. and Nikhil Karthik, R. and Ashwini Prasad and Dhananjaya, B. L. and Satish, S. and Prasad, Hara and Nagendra Prasad, M. N. (2017) Phytogenic synthesis of nanoparticles from Rhizophora mangle and their bactericidal potential with DNA damage activity. Nano-Structures & Nano-Objects, 10. 112 - 115. ISSN 2352-507X
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The present study reports phytogenic synthesis of nanoparticles using aqueous extract of Rhizophora mangle. The synthesized nanoparticles were characterized using UV–Visible spectroscopy with maximum absorption at 400 nm. Fourier Transform Infrared spectroscopy (FT-IR) studies revealed the presence of functional groups mediating the synthesis of nanoparticles. X-ray diffraction (XRD) displayed Bragg’s intensities at 2θ angle which confirmed the crystalline nature of the nanoparticles. Transmission Electron Microscopy (TEM) revealed size and shape of the nanoparticles with polydispersity of nanoparticles and size of 10–60 nm. The synthesized nanoparticles expressed antibacterial activity by well diffusion assay, broth micro dilution assay, minimal inhibitory concentration and CFU assay against selective human pathogens. Nanoparticles were more effective against S. aureus (MTCC 7443) followed by B. subtilis (MTCC 121), E. coli (MTCC 7410) and S. typhi (MTCC 7407). The possible mode of action of nanoparticles was studied by treating silver nanoparticles with pathogenic DNA which showed defamed and damage DNA in comparison with control DNA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Phytogenic,Nanoparticles, Bactericidal, DNA damage |
| Subjects: | B Life Science > Microbiology |
| Divisions: | Department of > Microbiology |
| Depositing User: | C Swapna Library Assistant |
| Date Deposited: | 10 Mar 2020 05:04 |
| Last Modified: | 19 Jul 2022 11:20 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/11893 |
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