Sensitive bromatometric assay methods for finasteride in pharmaceuticals.

Basavaiah, K. and Somashekar, B. C. and Anil Kumar, U. R. and Ramakrishna, V. (2006) Sensitive bromatometric assay methods for finasteride in pharmaceuticals. Ecletica Quimica, 31 (3, Cop). pp. 31-37. ISSN 0100-4670

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Three sensitive spectrophotometric methods are presented for the detn. of finasteride in bulk and in tablets. The methods rely on the use of bromate-bromide reagent and 3 dyes namely, methylorange, indigo carmine and thymol blue as reagents. They involve the addn. of a measured excess of bromate-bromide reagent to finasteride in acid medium, and after the bromination reaction is judged to be complete, the unreacted bromine is detd. by reacting with a fixed amt. of either methylorange and measuring the absorbance at 520 nm (method A) or indigo carmine and measuring the absorbance at 610 nm (method B) or thymol blue and measuring the absorbance at 550 nm (method C). In all the methods, the amt. of in situ generated bromine reacted corresponds to the amt. of finasteride. The absorbance measured at the resp. wavelength increased linearly with the concn. of finasteride. Beer's law was obeyed in the ranges 0.25-2.0, 0.5-6.0 and 1-12 μg mL-1 for methods A, B and C, resp. The calcd. molar absorptivity values were 5.7 × 104, 3.12 × 104 and 1.77 × 104 L mol-1 cm-1, resp., for method A, method B and method C, and the corresponding Sandell sensitivity values were 0.0065, 0.012 and 0.021 μg cm-2. The limits of detection and quantification were also reported for all the methods. Accuracy and, intraday and inter-day precisions of the methods were established according to the current ICH guidelines. The methods were successfully applied to the detn. of finasteride in com. available tablets and the results closely agreed with the label claim. The results of the methods were statistically compared with those of a ref. method by applying Student's t-test and F-test. The accuracy and reliability of the methods were further confirmed by performing recovery tests via std. addn. procedure. [on SciFinder(R)]

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2006/// [EPrints field already has value set] M3 - 10.1590/S0100-46702006000300004 [Field not mapped to EPrints] JA - Ecletica Quim. [Field not mapped to EPrints]
Uncontrolled Keywords: bromatometric assay finasteride pharmaceutical
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Dhruvakumar
Date Deposited: 03 Jun 2013 07:08
Last Modified: 06 Sep 2013 05:33

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