No role of poly(ADP-ribose) polymerase in EMS induced adaptive response in meiotic cells of Poecilocerus pictus.

Guruprasad, K. P. and Vasudev, V. and Harish, S. K. and Venu, R. (1999) No role of poly(ADP-ribose) polymerase in EMS induced adaptive response in meiotic cells of Poecilocerus pictus. Schriften des Forschungszentrums Juelich, Bilateral Seminars of the International Bureau, 30 (Recent). pp. 153-157. ISSN 1433-5581

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Recently the authors have reported the existence of adaptive response of an in vivo animal systems such as P. pictus and mouse and in vitro system of human lymphocytes. Different repair enzymes have been implicated in the process of adaptive response. In an attempt to understand the involvement of poly(ADP-ribose) polymerase (PARP) in ethylmethanesulfonate (EMS) induced adaptive response of P. pictus, present investigations were undertaken by employing nicotinamide, an inhibitor of PARP. Inter-, pre-, and post-treatment expts. were carried out using meiotic cells of P. pictus. In inter-treatment expts. animals were treated with conditioning dose of EMS (0.03 M). One hour later they were injected with nicotinamide (5 mM) and then challenged after one hour with 0.12 M of EMS. In the pre- and post-treatments, nicotinamide was given 2 or 4 h before or after combined treatment (conditioning+challenging), resp. In all cases animals were sacrificed at 12, 24, 36, and 48 h recovery times. Regular squash prepns. were made from the testes and the stained slides were screened for the presence of anomalies at metaphase I and II and anaphase I and II. It is very interesting to note that in all cases there were significant redn. in the chromosomal anomalies compared to resp. combined treatment. From these results we can conclude that: (a) nicotinamide acts as a potentiator of inducible adaptive response and (b) PARP is not involved in the inducible adaptive response in the meiotic cells of P. pictus. [on SciFinder(R)]

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 1999/// [EPrints field already has value set] JA - Schr. Forschungszent. Juelich, Bilateral Semin. Int. Bur. [Field not mapped to EPrints]
Uncontrolled Keywords: polyADPribosepolymerase ethyl methanesulfonate nicotinamide meiose adaptation Poecilocerus
Subjects: Agriculture Biological Sciences > Zoology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Zoology
Divisions: PG Campuses > Manasagangotri, Mysore > Zoology
Depositing User: Kruthi M.S
Date Deposited: 01 Jun 2013 07:59
Last Modified: 01 Jun 2013 07:59

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