Molecular profiling and antimicrobial potential of endophytic Gliomastix polychroma CLB-32 inhabiting Combretum latifolium Blume

Rao, H. C. Y and Baker, S. and Rakshith, D. and Satish, S. (2015) Molecular profiling and antimicrobial potential of endophytic Gliomastix polychroma CLB-32 inhabiting Combretum latifolium Blume. Mycology. ISSN 2150-1211

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Abstract

Fungal endophytes as a source of bioactive metabolites have led to the development of pharmaceutical products finding new applications. In a survey of endophytic fungal biodiversity, an antimicrobial endophytic strain CLB32 was isolated from the leaf of Combretum latifolium Blume (Combretaceae) from the Western Ghats of Southern India. CLB32 was then identified as Gliomastix polychroma (KR704576) by morphological and phylogenetic analysis based on internal transcribed spacer (ITS) nuclear rDNA and intervening 5.8S rRNA gene. CLB32 here constituted the first report on incidence of endophytic fungi from C. latifolium Blume. Ethyl acetate fraction of strain CLB32 was evaluated for antimicrobial activity by disc diffusion assay. Secondary metabolites produced effectively inhibited methicillin-resistant Staphylococcus aureus (18.33 ± 0.33 mm), Pseudomonas aeruginosa (14.66 ± 0.33 mm) and Candida albicans (14.00 ± 0.57 mm). Biosynthesis of these antimicrobial compounds was detected by analytical TLC-bioautography method as depicted by zone of inhibition on intensive the band. These findings suggest that G. polychroma CLB32, as a producer of natural antimicrobial drugs, could help to combat against multidrug-resistant infections and also provide baseline information for industrial applications.

Item Type: Article
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: lpa manjunath user
Date Deposited: 07 Mar 2020 06:11
Last Modified: 28 Dec 2020 06:10
URI: http://eprints.uni-mysore.ac.in/id/eprint/11489

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