Simple, sensitive and selective spectrophotometric methods for the determination of methdilazine in pharmaceuticals through charge transfer complex formation reaction

Raghu, M. S. and Basavaiah, K. (2013) Simple, sensitive and selective spectrophotometric methods for the determination of methdilazine in pharmaceuticals through charge transfer complex formation reaction. Proceedings of the Indian National Science Academy, 79 (1). pp. 65-78.

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Abstract

Three rapid, selective and sensitive spectrophotometric methods are described for the quantitative determination of methdilazine hydrochloride (MDH) in pure form as well as in its pharmaceutical formulations. The methods are based on charge transfer complexation reaction of base form of MDH as n-electron donor with 2, 3-Dichloro-5, 6-dicyano-1,4- benzoquinone (DDQ), 2,4-Dinitrophenol (DNP) and 2,4, 6-Trinitrophenol (picric acid; PA) as p-acceptors to give highly coloured radical anion species. The coloured products were quantified spectrophotometrically at 585 nm with DDQ (method A), at 420 nm with DNP (method B) and at 425 nm with PA (method C). Under the optimized experimental conditions, Beer�s law is obeyed over the concentration ranges of 4-64, 2.5-40 and 2-32 ìg ml-1 MDZ for method A, method B and method C, respectively. The molar absorptivity, Sandell sensitivity, limits of detection and quantification are also reported. The effects of reaction medium, reaction time and reagent concentration on the sensitivity and stability of the complexes formed have been examined. The proposed methods were successfully applied to the determination of MDH in pure form and commercial tablets and syrup with good accuracy and precision. Statistical comparison of the results was performed using Student�s t-test and F-ratio at 95 confidence level and the results showed no significant difference between the reference and proposed methods with regard to accuracy and precision. Further, the accuracy and reliability of the methods were confirmed by recovery studies via standard addition technique.

Item Type: Article
Uncontrolled Keywords: Methdilazine and Determination and Charge-Transfer Complexes and Spectrophotometry and Pharmaceuticals
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Arshiya Kousar
Date Deposited: 22 Jan 2020 05:54
Last Modified: 22 Jan 2020 05:54
URI: http://eprints.uni-mysore.ac.in/id/eprint/10806

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