Simple and sensitive spectrophotometric determination of ganciclovir using bromate-bromide, M-cresol purple and erioglaucine

Ramesh, P. J. and Basavaiah, K. and Xavier, C. M. and Prashanth, K. N. and Raghu, M. S. and Vinay, K. B. (2013) Simple and sensitive spectrophotometric determination of ganciclovir using bromate-bromide, M-cresol purple and erioglaucine. Proceedings of the Indian National Science Academy, 79 (1). pp. 1-9.

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Two spectrophotometric methods, which are simple and sensitive, are described for the determination of ganciclovir (GNC) in bulk drug and in its formulations. The methods use bromate-bromide, m-cresol purple and erioglaucine as reagents. GNC is treated with a known excess of bromate-bromide mixture in acid medium and after the bromination reaction is ensured to be complete, the unreacted bromine is estimated by treating with a fixed amount of either m-cresol purple and measuring the absorbance at 540 nm (method A) or erioglaucine and measuring the absorbance at 630 nm (method B). In both methods, the amount of bromate that reacted corresponds to the drug content. The calibration graphs were found to be linear over 0.5-10 and 0.6-7.5 ìg mL�1 for method A and method B, respectively, with corresponding molar absorptivity values of 1.86 x105 and 2.29 x 104 L mol�1 cm�1. The Sandell sensitivity values, limits of detection (LOD) and quantification (LOQ) are also reported. Accuracy and precision of the assays were determined by computing the intra-day and inter-day variations at three different levels of GNC; the intra-day and inter-day RSD were < 2.66 and the accuracy was better than 2.49. The methods were successfully applied to the determination of GNC in two different brands of tablets with good accuracy and precision and without detectable interference by excipients. The accuracy was further ascertained by placebo blank and synthetic mixture analyses and also by recovery experiments via standardaddition procedure.

Item Type: Article
Uncontrolled Keywords: Ganciclovir and Determination and Titrimetry and Spectrophotometry and Pharmaceuticals
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 21 Jan 2020 06:07
Last Modified: 21 Jan 2020 06:07

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