Utilization of 3-ethyl-1(N,N-dimethyl)aminopropylcarbodiimide (EDCI)/1-hydroxybenzotriazole (HOBt) as a polymerizing agent

Baba, A. R. and Gowda, D. C. (2001) Utilization of 3-ethyl-1(N,N-dimethyl)aminopropylcarbodiimide (EDCI)/1-hydroxybenzotriazole (HOBt) as a polymerizing agent. In: UNSPECIFIED Kluwer Academic Publ, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS, pp. 309-318.

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Abstract

The commonly used coupling reagents in peptide syntheses such as dicyclohexylcabodiimide, diisopropylcarbodiimide and 3-ethyl-1(N,N-dimethyl) aminopropylcarbodiimide with or without 1-hydroxybenzotriazole or N-hydroxysuccinimide have been used as polymerizing agents in the synthesis of elastic/plastic protein-based polymers. It was found that 3-ethyl-1(N,N-dimethyl) aminopropylcarbodiimide with 1-hydroxybenzotriazole gave equally good polymers comparable to conventional p-nitrophenol approach. Further, we present here the polymerization and characterization of structural and functional properties of poly(Val-Pro-Gly-Val-Gly), which is the most striking repeating sequence in the bovine and porcine elastins. The polymers obtained by both p-nitrophenol and 3-ethyl-1(N,N-dimethyl) aminopropylcarbodiimide approach were characterized by carbon-13 and proton nuclear magnetic resonance spectroscopy, differential scanning calorimetry, circular dichroism and fourier transform infrared spectroscopy. These results conclude that poly(Val-Pro-Gly-Val-Gly) obtained by both methods were identical in all respects of physical and chemical properties indicates that 3-ethyl-1(N,N-dimethyl) aminopropylcarbodiimide with 1-hydroxybenzotriazole method can be conveniently employed to synthesize these polymers.

Item Type: Book Section
Uncontrolled Keywords: beta-turn; 3-ethyl-1(N,N-dimethyl)aminopropylcarbodiimide; conformational analysis; inverse temperature transition; polymerization; thermal analysis
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Harisha R uom
Date Deposited: 31 Jan 2020 06:06
Last Modified: 01 Jan 2021 04:46
URI: http://eprints.uni-mysore.ac.in/id/eprint/10139

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