Role of catalytic function in the antiplatelet activity of phospholipase A(2) cobra (Naja naja naja) venom

Rudrammaji, L. M. S. and Machiah, K. D. and Kantha, T. P. K. and Veerabasappa Gowda, T. (2001) Role of catalytic function in the antiplatelet activity of phospholipase A(2) cobra (Naja naja naja) venom. Molecular and Cellular Biochemistry, 219 (1-2). pp. 39-44.

Text (Full Text) Rudrammaji2001_Article_RoleOfCatalyticFunctionInTheAn.pdf Download (282kB)

Abstract

Three acidic phospholipases A(2) from Indian cobra (Naja naja naja) venom inhibited platelet aggregation in platelet rich plasma induced separately by ADP, collagen and epinephrine with different potencies. The order of inhibition was epinephrine > collagen > ADP. They did not inhibit platelet aggregation induced by arachidonic acid (10 muM). The inhibition was dependent on concentration of the protein and the time of incubation of the phospholipases A(2) with platelet rich plasma. Parabromophenacyl bromide modified PLA(2) enzymes lost their enzymatic activity as well as platelet aggregation inhibition activity suggesting the involvement of catalytic function in platelet aggregation inhibitory activity.

Item Type:	Article
Uncontrolled Keywords:	platelet aggregation inhibitors; acicid phospholipases; antiplatelet activity; catalytic function; phospholipases A2
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Harisha R uom
Date Deposited:	19 Apr 2021 09:34
Last Modified:	12 Jul 2022 08:00
URI:	http://eprints.uni-mysore.ac.in/id/eprint/10120

Actions (login required)

View Item